Khalil, H., Hassaneine, H., El-Sheikh, A., Abdelbary, A. (2021). Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats. Zagazig Journal of Forensic Medicine and Toxicology, 19(2), 31-48. doi: 10.21608/zjfm.2021.71515.1074
Heba Khalil; Hanan Hassaneine; Arwa Ahmed El-Sheikh; Abeer Mohamed Abdelbary. "Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats". Zagazig Journal of Forensic Medicine and Toxicology, 19, 2, 2021, 31-48. doi: 10.21608/zjfm.2021.71515.1074
Khalil, H., Hassaneine, H., El-Sheikh, A., Abdelbary, A. (2021). 'Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats', Zagazig Journal of Forensic Medicine and Toxicology, 19(2), pp. 31-48. doi: 10.21608/zjfm.2021.71515.1074
Khalil, H., Hassaneine, H., El-Sheikh, A., Abdelbary, A. Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats. Zagazig Journal of Forensic Medicine and Toxicology, 2021; 19(2): 31-48. doi: 10.21608/zjfm.2021.71515.1074
Evaluation of The Role of Buffalo Milk on Acute Toluene Induced Hepatotoxicity and Nephrotoxicity in Adult Male Albino Rats
1forensic and clinical toxicology department ,faculty of medicine ,zagazig university
2department of forensic medicine and clinical toxicology , faculty of medicine ,zagazig university
3Department of Forensic Medicine and Clinical toxicology
4department of pathology,faculty of medicine,zagazig university.
Abstract
Toluene is widely used all over the world as a volatile organic compound and manufactured in big amounts for use in various applications including paints, inks, adhesives, varnishes and plastics. Aim of the work: This study was implemented to evaluate the role of buffalo milk in ameliorating toxic effects of toluene in adult male albino rats. Methodology:72 adult male albino rats were used in this study.They were divided into five groups as follow: Group I (negative control) 12 rats received regular diet and water to measure basic parameters.Group II (positive control) 24 rats subdivided into 2 equal groups: Subgroup IIa :12 rats received 1 ml of corn oil . Subgroup IIb : 12 rats received 1ml of distelled water . Group III (pasturalized buffalo milk group (PBM)12 rats received 1ml of buffalo milk .Group IV (toluene group)12 rats received toluene 900mg/kg dissolved in corn oil . Group V (toluene and pasturalized buffalo milk group) 12 rats received toluene 900mg/kg followed by 1ml of buffalo milk for 7 days. At the end of the study, blood samples were collected for estimating (serum ALP, ALT, albumin, creatinine, MDA, TAC). Then livers and kidneys were extracted for histopathological and immunohistochemistry (caspase-3) examination. Results: The result of the study revealed that administration of toluene for 7 days induced significant increase in ALP, ALT, creatinine, serum MDA and significant reduction in serum albumin and TAC. It also induced histopathological alterations in liver and kidney with increased caspase-3 immunoreactivity. Administration of PBM with toluene for 7 days improved toluene induced histopathological alterations in liver and kidney with decreased caspase-3 immunoreactivity. Also, PBM caused significantly beneficial effect on liver and kidney parameters; In addition, it decreased serum MDA level and increased serum TAC. Conclusion: Buffalo milk has partial protective effects against toluene induced hepatotoxicityand nephrotoxicity,
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