Madboly, A., Adrees, H., Abd Al-Aziz, S., Alhusseini, N., Haggag, O. (2021). DNA Fingerprinting for Determination of Phylogenetic Tree: A Genomic Study on Samples of Egyptian and Iraqi Populations. Zagazig Journal of Forensic Medicine and Toxicology, 19(1), 74-90. doi: 10.21608/zjfm.2020.34578.1059
Abdelmonem G. Madboly; Hussein A. Adrees; Samer H. Abd Al-Aziz; Naglaa F. Alhusseini; Ola G. Haggag. "DNA Fingerprinting for Determination of Phylogenetic Tree: A Genomic Study on Samples of Egyptian and Iraqi Populations". Zagazig Journal of Forensic Medicine and Toxicology, 19, 1, 2021, 74-90. doi: 10.21608/zjfm.2020.34578.1059
Madboly, A., Adrees, H., Abd Al-Aziz, S., Alhusseini, N., Haggag, O. (2021). 'DNA Fingerprinting for Determination of Phylogenetic Tree: A Genomic Study on Samples of Egyptian and Iraqi Populations', Zagazig Journal of Forensic Medicine and Toxicology, 19(1), pp. 74-90. doi: 10.21608/zjfm.2020.34578.1059
Madboly, A., Adrees, H., Abd Al-Aziz, S., Alhusseini, N., Haggag, O. DNA Fingerprinting for Determination of Phylogenetic Tree: A Genomic Study on Samples of Egyptian and Iraqi Populations. Zagazig Journal of Forensic Medicine and Toxicology, 2021; 19(1): 74-90. doi: 10.21608/zjfm.2020.34578.1059
DNA Fingerprinting for Determination of Phylogenetic Tree: A Genomic Study on Samples of Egyptian and Iraqi Populations
1Department of Forensic Medicine & Clinical Toxicology; Faculty of Medicine, Benha University, Egypt
2Biology, Science College, Al-Muthanna University
3Botany and Microbiology, Faculty of Science, Benha University, Egypt
4Medical Biochemistry & Molecular Biology, Faculty of Medicine, Benha University, Egypt
5Forensic Medicine & Clinical Toxicology department, Faculty of Medicine, Benha University, Egypt
Abstract
ABSTRACT
Background: Phylogenetic analysis is widely used to determine the evolutionary relationships, and it is expressed by the phylogenetic tree. The current work aimed to identify the phylogenetic tree of Egyptian and Iraqi population samples, and to compare between the used deoxyribonucleic acid (DNA) fingerprinting techniques [Inter-Simple Sequence Repeat (ISSR)- polymerase chain reaction (PCR) and Random Amplified Polymorphic DNA (RAPD)-PCR]. Methodology: This comparative cross-sectional study was carried out on 48 unrelated healthy volunteers from different geographic areas [Northern Egypt “NE”, Southern Egypt “SE”, Northern Iraq “NI” and Southern Iraq “SI”]; twelve volunteers from each area. Seven primers (OP-A3, OP-A9, OP-B3, OP-C3, OP-D1, OP-C15 and OP-K2) were used in RAPD-PCR. Seven primers (14A, 44B, HB-9, HB-10, HB-11, HB-12 and HB-13) were used in ISSR-PCR. Results: Similarity coefficients and phylogenetic trees revealed the genetic distance and relationship of the studied groups as follows: the highest genetic overlap “similarity” was between SE and both NI and NE, respectively. The biggest genetic distance (variation) was between SI and both “NI and NE”. The study concluded that: (1) RAPD-PCR method was more valuable in detecting genetic polymorphism as compared to ISSR-PCR method, (2) Combined analysis using both RAPD and ISSR-PCR methods gave a clear indication of genetic variations than either RAPD or ISSR-PCR techniques.
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