Elnajjar, N., Mokhtar, M., Habashy, O., Attallah, H., Abdelrahman, S., Elsharkawey, S. (2023). Estimation of Tissue Homogenate Cytokines and MicroRNAs might Help to Determine Wound Vitality and Dating. Zagazig Journal of Forensic Medicine and Toxicology, 21(1), 157-171. doi: 10.21608/zjfm.2023.182949.1136
Noha Elnajjar; Maha M. Mokhtar; Omnia Habashy; Hebatallah E. Attallah; Shaymaa M. Abdelrahman; Sally Elsharkawey. "Estimation of Tissue Homogenate Cytokines and MicroRNAs might Help to Determine Wound Vitality and Dating". Zagazig Journal of Forensic Medicine and Toxicology, 21, 1, 2023, 157-171. doi: 10.21608/zjfm.2023.182949.1136
Elnajjar, N., Mokhtar, M., Habashy, O., Attallah, H., Abdelrahman, S., Elsharkawey, S. (2023). 'Estimation of Tissue Homogenate Cytokines and MicroRNAs might Help to Determine Wound Vitality and Dating', Zagazig Journal of Forensic Medicine and Toxicology, 21(1), pp. 157-171. doi: 10.21608/zjfm.2023.182949.1136
Elnajjar, N., Mokhtar, M., Habashy, O., Attallah, H., Abdelrahman, S., Elsharkawey, S. Estimation of Tissue Homogenate Cytokines and MicroRNAs might Help to Determine Wound Vitality and Dating. Zagazig Journal of Forensic Medicine and Toxicology, 2023; 21(1): 157-171. doi: 10.21608/zjfm.2023.182949.1136
Estimation of Tissue Homogenate Cytokines and MicroRNAs might Help to Determine Wound Vitality and Dating
1Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University
2Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Benha University
3Medical Biochemistry and Molecular Biology, Faculty of Medicine, Benha University
Abstract
Objectives: To assess the applicability of estimated levels of tumor necrosis factor-α (TNF-α) and interleukin (IL)-6, and gene expression levels of microRNAs (Mir) 92a and 214a in tissue homogenate (TH) of skin biopsies harvested from wound for discrimination between antemortem and post-mortem wounds and to suggest the post-injury interval (PII). Experimental Protocol: A 2-cm skin incision was made under anesthesia and full thickness punches were obtained from wound edge immediately (C-group) and at 30-min, 2-h, 6-h and 24-h after wounding in living animals (L-group) or animals were decapitated immediately after wounding and biopsies were obtained at the same periods after decapitation (D-group). Tissues were homogenized to be used for ELISA estimation of TNF-α and IL-6 levels and qRT-PCR expression levels of Mir-92a and 214a. Results: TNF-α, IL-6 and Mir-92a levels were significantly higher in L-group than other groups. Estimated TNF-α and IL-6 levels showed biphasic increases at 30-min and 2h, respectively and at 24h for both, while the peak levels of mir-214a and mir-92a were at 2h and 6h, respectively. MicroRNAs levels showed non-significant differences between all D-group specimens. Regression analysis defined high IL-6 levels as the significant variate to identify PII as either 2h or 24h and high levels of Mir-214a could suggest PII of 2h, while high levels of Mir-92a and TNF-α as the significant variate to suggest PII of 30-min and 6h, respectively. Multivariate analysis defined high IL-6 as the persistently significant predictor for victim's vitality at wounding, while ROC curve analysis defined high Mir-214a levels as the sensitive identifier for victim's viability during wounding. Conclusion: Estimation of expression levels of Mir-92a and 214a in TH might define the probable PII and differentiate antemortem from postmortem wounds, respectively. Combined markers might increase the accuracy of wound-dating.
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