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Abdel Wahab, D., Abd Allah, S., Ibrahim, N., Meleka, H. (2018). Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice. Zagazig Journal of Forensic Medicine and Toxicology, 16(2), 17-24. doi: 10.21608/zjfm.2019.3037.1011
Dena Abdel Wahab; Somia Abd Allah; Nermein Ibrahim; Hany Meleka. "Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice". Zagazig Journal of Forensic Medicine and Toxicology, 16, 2, 2018, 17-24. doi: 10.21608/zjfm.2019.3037.1011
Abdel Wahab, D., Abd Allah, S., Ibrahim, N., Meleka, H. (2018). 'Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice', Zagazig Journal of Forensic Medicine and Toxicology, 16(2), pp. 17-24. doi: 10.21608/zjfm.2019.3037.1011
Abdel Wahab, D., Abd Allah, S., Ibrahim, N., Meleka, H. Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice. Zagazig Journal of Forensic Medicine and Toxicology, 2018; 16(2): 17-24. doi: 10.21608/zjfm.2019.3037.1011

Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice

Article 2, Volume 16, Issue 2, July 2018, Page 17-24  XML PDF (591.78 K)
Document Type: Original Article
DOI: 10.21608/zjfm.2019.3037.1011
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Authors
Dena Abdel Wahab email orcid 1; Somia Abd Allah2; Nermein Ibrahim3; Hany Meleka4
1Forensic medicine and toxicology department Faculty of medicine Zagazig university
2Biochemistry department Faculty of medicine zagazig university
3Forensic and toxicology department Faculty of medicine zagazig university
4Forensic medicine and toxicology Faculty of medicine Zagazig university
Abstract
Introduction: Postmortem personal identification is based on the extraction of deoxyribonucleic acid (DNA) from human remains or different human materials. There are some reports proving that various tissues may differ genetically and do not always have the DNA profile of the person from whom they originate due to mutation or chimerism. Aim of the work: The work aimed at the postmortem detection of the possible change in DNA fingerprint after allogeneic hematopoietic stem cell transplantation in liver tissue samples. Materials and Methods: One hundred twenty eight adult male mice weighing 25g. DNA fingerprint first was done from blood samples. After 3 months from interference, postmortem DNA fingerprinting was done again from liver tissue. Results: Fluorescent stem cells were detected postmortem in liver tissue of 30 mice by percentage of 93.75%. The change in DNA profile was detected postmortem in 20 mice in liver tissue samples by a percentage of 62.5%. Conclusion: DNA chimerism, after allogeneic hematopoietic stem cell transplantation, can be detected postmortem in the recipient's liver. Recommendations: It's recommended to study the postmortem detection of DNA chimerism in organs other than the liver to compare the percentage of chimerism detection.
Key words: Postmortem; Liver; Chimerism; Allogeneic Hematopoietic Stem Cell Transplantation.
Keywords
Postmortem; Liver; Chimerism; Allogeneic Hematopoietic Stem Cell Transplantation
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