Postmortem Detection of Liver Chimerism after Allogeneic Hematopoietic Stem Cell Transplantation in Adult Male Mice

Document Type : Original Article

Authors

1 Forensic medicine and toxicology department Faculty of medicine Zagazig university

2 Biochemistry department Faculty of medicine zagazig university

3 Forensic and toxicology department Faculty of medicine zagazig university

4 Forensic medicine and toxicology Faculty of medicine Zagazig university

Abstract

Introduction: Postmortem personal identification is based on the extraction of deoxyribonucleic acid (DNA) from human remains or different human materials. There are some reports proving that various tissues may differ genetically and do not always have the DNA profile of the person from whom they originate due to mutation or chimerism. Aim of the work: The work aimed at the postmortem detection of the possible change in DNA fingerprint after allogeneic hematopoietic stem cell transplantation in liver tissue samples. Materials and Methods: One hundred twenty eight adult male mice weighing 25g. DNA fingerprint first was done from blood samples. After 3 months from interference, postmortem DNA fingerprinting was done again from liver tissue. Results: Fluorescent stem cells were detected postmortem in liver tissue of 30 mice by percentage of 93.75%. The change in DNA profile was detected postmortem in 20 mice in liver tissue samples by a percentage of 62.5%. Conclusion: DNA chimerism, after allogeneic hematopoietic stem cell transplantation, can be detected postmortem in the recipient's liver. Recommendations: It's recommended to study the postmortem detection of DNA chimerism in organs other than the liver to compare the percentage of chimerism detection.
Key words: Postmortem; Liver; Chimerism; Allogeneic Hematopoietic Stem Cell Transplantation.

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