Elsayed, M., Hassan, M., Abaza, M. (2023). Role of Clove Essential Oil on Benzo(a)pyrene Induced Lung Toxicity in Adult Male Albino Rats. Zagazig Journal of Forensic Medicine and Toxicology, 21(2), 115-130. doi: 10.21608/zjfm.2023.193148.1140
Manar Ali Elsayed; Maha Zayed Mohammed Hassan; Marwa Tawfeek Abaza. "Role of Clove Essential Oil on Benzo(a)pyrene Induced Lung Toxicity in Adult Male Albino Rats". Zagazig Journal of Forensic Medicine and Toxicology, 21, 2, 2023, 115-130. doi: 10.21608/zjfm.2023.193148.1140
Elsayed, M., Hassan, M., Abaza, M. (2023). 'Role of Clove Essential Oil on Benzo(a)pyrene Induced Lung Toxicity in Adult Male Albino Rats', Zagazig Journal of Forensic Medicine and Toxicology, 21(2), pp. 115-130. doi: 10.21608/zjfm.2023.193148.1140
Elsayed, M., Hassan, M., Abaza, M. Role of Clove Essential Oil on Benzo(a)pyrene Induced Lung Toxicity in Adult Male Albino Rats. Zagazig Journal of Forensic Medicine and Toxicology, 2023; 21(2): 115-130. doi: 10.21608/zjfm.2023.193148.1140
Role of Clove Essential Oil on Benzo(a)pyrene Induced Lung Toxicity in Adult Male Albino Rats
1Forensic Medicine and Clinical Toxicology Department, Faculty of Medicine, Zagazig University
2Histology and Cell Biology Department, Faculty of Medicine, Zagazig University
3Forensic Medicine and Clinical Toxicology Department,Faculty of Medicine, Zagazig University
Abstract
Background: Environmental exposuretobenzo(a)pyrene (BP) elicit pulmonary inflammation and impair lung functions. Clove oil (CLV) possesses antioxidant, anti-inflammatory besides anticancer effects. The aim of this study: To evaluate the possible protecting role of clove oil (CLV) against lung injuries induced by benzo(a)pyrene (BP) in albino rats. Material and methods: Thirty rats were spilt randomly into 4 groups, control group (12 rats were divided equally into 2 smaller groups; negative and corn oil) as well as 3 experimental groups (each with 6 rats) (BP group: received a daily dose of (50 mg/kg) orally for 4 weeks, CLV group: received a daily dose of (100 µl) orally for 4 weeks, and CLV/BP group: received both CLV and BP in the same doses orally for 4 weeks). After 4 weeks, blood specimens then lung tissue were collected. Lactate dehydrogenase (LDH), reduced glutathione (GSH), Malondialdehyde (MDA), plus cancer marker carcinoembryonic antigen (CEA) were determined. Inflammatory mediators interleukin-1β (IL-1β) besides tumour necrosis factor-α (TNF-α) were estimated in lung tissues. Histopathological examination by hematoxyline and eosin stain plus immunohistochemical examination of proliferating cell nuclear antigen (PCNA) expression were evaluated. Results: GSH was decreased but LDH, MDA, IL-1β, TNF-α and CEA were elevated in BP treated group. Clove oil co-administration ameliorated the biochemical parameters and the histopathological alterations in lung tissues. Also, it reduced the PCNA expression caused by BP in the immunohistochemical examination. Conclusion: Clove oil might ameliorate BP elicited pulmonary dysfunction. Recommendations: Additional studies are required to explain the molecular mechanisms fundamental to clove oil antitumor activity.
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