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Abdelhady, R., thabet, H., yassa, H., Ebrahem, N. (2019). Role of aging in identification of DNA extracted from blood and seminal stains. Zagazig Journal of Forensic Medicine and Toxicology, 17(2), 19-33. doi: 10.21608/zjfm.2019.12124.1029
Randa Hussien Abdelhady; hayam zakaria thabet; heba atia yassa; Noha Esmael Ebrahem. "Role of aging in identification of DNA extracted from blood and seminal stains". Zagazig Journal of Forensic Medicine and Toxicology, 17, 2, 2019, 19-33. doi: 10.21608/zjfm.2019.12124.1029
Abdelhady, R., thabet, H., yassa, H., Ebrahem, N. (2019). 'Role of aging in identification of DNA extracted from blood and seminal stains', Zagazig Journal of Forensic Medicine and Toxicology, 17(2), pp. 19-33. doi: 10.21608/zjfm.2019.12124.1029
Abdelhady, R., thabet, H., yassa, H., Ebrahem, N. Role of aging in identification of DNA extracted from blood and seminal stains. Zagazig Journal of Forensic Medicine and Toxicology, 2019; 17(2): 19-33. doi: 10.21608/zjfm.2019.12124.1029

Role of aging in identification of DNA extracted from blood and seminal stains

Article 2, Volume 17, Issue 2 - Serial Number 17, July 2019, Page 19-33  XML PDF (913.04 K)
Document Type: Original Article
DOI: 10.21608/zjfm.2019.12124.1029
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Authors
Randa Hussien Abdelhady1; hayam zakaria thabet2; heba atia yassa3; Noha Esmael Ebrahem email orcid 4
1Department of Forensic Medicine & Clinical Toxicology, Faculty of Medicine, Assiut University
2forensic medicine and clinical toxicology department, faculty of medicine , Assuit university , egypt
3forensic medicine and clinical toxicology,faculty of medicine, Assuit university,Assuit ,Egypt
4forensic medicine and clinical toxicology , faculty of medicine, assiut university
Abstract
DNA investigation is playing a vital role than ever before in criminal cases as samples retrieved from crime scene may be exposed to different conditions before proceeding that can lead to degradation of DNA.One of these conditions is the aging effect. The aim of this study is to evaluate the effect of aging process on DNA extraction and typing from blood and seminal stains.The present study was done on twenty male volunteers after written consent; four ml of blood and two ml of semen were collected from the same volunteer and spotted on pieces of cotton fabric. The effect of aging (one, three and six months) extraction of DNA from blood and seminal stains were tested. DNA concentration was measured by spectrophotometer and analyzed by using gel electrophoresis. It was shown that blood and seminal stains left at room temperature for one, three and six months can be identified using presumptive tests. DNA concentration was significantly reduced in blood and seminal stains left for one, three and six months before processing. Quality of DNA was not affected by storage length and DNA fragments could be identified except for bloodstains left for six months which cannot detected by Amelogenin primer. In conclusion; DNA can be extracted from blood and seminal stains left for one, three and six months in room temperature. It is recommended to expose the samples to longer periods more than 6 months at room temperature. Different primers can be used for further research. Other types of samples can be used in further studies rather than blood and semen as saliva and teeth.
Keywords
Blood; Semen; Degradation; DNA; Amelogenin; primers
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