EL-shafey, R., Fakher, H., Abdelhady, L., Hussein, A. (2025). Role of Autophagy (BECLIN -1) and Apoptotic (BAX) Associated Proteins in Discrimination between Antemortem and Postmortem Burn injuries in Rats Skin Biopsies. Zagazig Journal of Forensic Medicine and Toxicology, 23(1), 90-100. doi: 10.21608/zjfm.2024.325887.1202
Rabab EL-shafey; Haidy Fakher; Lina Mohamed Abdelhady; Asmaa Hussein. "Role of Autophagy (BECLIN -1) and Apoptotic (BAX) Associated Proteins in Discrimination between Antemortem and Postmortem Burn injuries in Rats Skin Biopsies". Zagazig Journal of Forensic Medicine and Toxicology, 23, 1, 2025, 90-100. doi: 10.21608/zjfm.2024.325887.1202
EL-shafey, R., Fakher, H., Abdelhady, L., Hussein, A. (2025). 'Role of Autophagy (BECLIN -1) and Apoptotic (BAX) Associated Proteins in Discrimination between Antemortem and Postmortem Burn injuries in Rats Skin Biopsies', Zagazig Journal of Forensic Medicine and Toxicology, 23(1), pp. 90-100. doi: 10.21608/zjfm.2024.325887.1202
EL-shafey, R., Fakher, H., Abdelhady, L., Hussein, A. Role of Autophagy (BECLIN -1) and Apoptotic (BAX) Associated Proteins in Discrimination between Antemortem and Postmortem Burn injuries in Rats Skin Biopsies. Zagazig Journal of Forensic Medicine and Toxicology, 2025; 23(1): 90-100. doi: 10.21608/zjfm.2024.325887.1202
Role of Autophagy (BECLIN -1) and Apoptotic (BAX) Associated Proteins in Discrimination between Antemortem and Postmortem Burn injuries in Rats Skin Biopsies
1Forensic Medicine and Clinical Toxicology department,Faculty of Medicine. Banha Univeristy
2Department of Medical Biochemistry and Molecular biology, Faculty of Medicine, Banha University,
Abstract
Background: Determining whether burn injuries occurred during life is a crucial topic in forensic medicine. Objectives: This research was studying the expression of the autophagy (Beclin-1) and apoptotic (Bax) associated proteins in rats skin tissues after burn infliction and evaluating the possible application value of these changes in identifying antemortem and postmortem injury. Materials and methods: The 25 rats were divided into 5 groups: the control group, the 0-hour antemortem injury group, the 24-hour antemortem injury group, the 0-hour postmortem injury group, and the 24-hour postmortem injury group. Burn models were induced with a digital thermostat water bath kettle (100°C) for 10 sec. The changes in levels of the Beclin-1 and Bax proteins were detected by Western blotting and the ELISA assay method, respectively, along with histopathological evaluation. Results: There was a reduction in expression levels of Beclin-1 in antemortem injured groups at time intervals 0 and 24-hour, which was statistically significant in the 24-hour antemortem burned group as compared to other studied groups. Estimation of Beclin-1 in both 0- and 24-hour postmortem injured groups revealed an elevation that was found to be significant in the 24-hour postmortem injured group. Estimation of the changes in Bax levels after burn infliction among the studied groups illustrated an elevation in both 0-hour and 24-hour antemortem burned groups, which was significant in 24-hour antemortem injured rats as compared to other groups. Our results illustrated a significant difference in Bax level found between both antemortem burn-inflicted groups and that postmortem burned groups at 24-hour. Conclusion: determination of Beclin-1 and Bax-associated protein levels might be used as novel biomarkers for differentiation between antemortem and postmortem burn injuries in a forensic context.
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